Effects of acetonitrele in eluent on elution volume when RSpak KC-811 is used is shown. By adding 10% acetonitrile to the eluent, the elution time of organic acids with strong hydrophobicity can be shortened.
Substance | Elution Volume (mL) | |
---|---|---|
3 mM HClO4 aq./CH3CN=90/10 | 3 mM HClO4 aq. | |
Oxalic acid |
9.28
|
9.62
|
Maleic acid |
9.91
|
10.51
|
α-Ketoglutaric acid |
10.39
|
10.87
|
Citric acid |
10.99
|
11.71
|
Isocitric acid |
11.05
|
11.88
|
2-Ketogluconic acid |
11.18
|
11.52
|
Glucuronic acid |
11.29
|
11.71
|
Oxaloacetic acid |
11.40
|
11.77
|
Pyruvic acid |
11.42
|
11.76
|
Phosphoric acid |
11.43
|
11.27
|
Tartaric acid |
11.53
|
12.14
|
trans-Aconitic acid |
11.57
|
13.11
|
Galacturonic acid |
11.90
|
12.41
|
Malonic acid |
12.10
|
12.96
|
Gluconic acid |
12.24
|
12.78
|
Malic acid |
12.37
|
13.37
|
Glyoxylic acid |
12.70
|
13.26
|
α-Hydroxyglutaric acid |
12.71
|
14.02
|
Ascorbic acid |
12.71
|
13.88
|
Succinic acid |
13.77
|
15.79
|
Itaconic acid |
14.08
|
16.80
|
Fumaric acid |
14.14
|
17.16
|
Uric acid |
14.99
|
18.31
|
Glycolic acid |
15.12
|
16.26
|
Adipic acid |
15.16
|
20.17
|
Lactic acid |
15.23
|
16.67
|
2-Hydroxyisobutyric acid |
15.26
|
17.05
|
Mesaconic acid |
15.42
|
20.47
|
β-Hydroxypropionic acid |
15.61
|
17.18
|
Levulinic acid |
16.44
|
20.27
|
Formic acid |
16.59
|
17.86
|
Acetic acid |
17.32
|
19.41
|
Pyroglutamic acid |
18.02
|
20.99
|
Propionic acid |
18.82
|
22.54
|
Isobutyric acid |
20.13
|
25.59
|
n-Butyric acid |
20.87
|
27.61
|
Isovaleric acid |
22.16
|
32.28
|
HCO3- |
23.36
|
24.50
|
n-Valeric acid |
24.64
|
38.89
|
Columns : Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2 Eluent : 3 mM HClO4 aq./CH3CN=90/10, 3 mM HClO4 aq. Flow rate : 1.0 mL/min Column temp. : 50 °C
Sample Name Index
- 2-Hydroxyisobutyric acid
- 2-Ketogluconic acid
- Acetic acid
- Adipic acid
- Ascorbic acid, Vitamin C
- Citric acid
- D-Glucuronic acid
- Formic acid
- Fumaric acid
- Galacturonic acid
- Gluconic acid
- Glycolic acid
- Glyoxylic acid
- Hydrogencarbonate, HCO3-
- Isobutyric acid
- Isocitric acid
- Isovaleric acid
- Itaconic acid
- Lactic acid
- Levulinic acid
- Maleic acid
- Malic acid
- Malonic acid
- Mesaconic acid
- Oxalic acid
- Oxaloacetic acid
- Phosphoric acid
- Propionic acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
- Tartaric acid
- Uric acid
- n-Butyric acid
- n-Valeric acid
- trans-Aconitic acid
- α-Hydroxyglutaric acid
- α-Ketoglutaric acid
- β-Hydroxypropionic acid, Hydracrylic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Beer (KC-811)
- Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Gluconic Acids (DE-613)
- Sake (Japanese Rice Wine) (KC-811)
- Organic Acids in White Wine (KC-811)
- Oxalic Acid (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Soy Source (KC-811)
- Organic Acids in Vinegar (KC-811)
- Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Organic Acids in Soybean Paste (KC-811)
- Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)