Effects of acetonitrele in eluent on elution volume when RSpak KC-811 is used is shown. By adding 10% acetonitrile to the eluent, the elution time of organic acids with strong hydrophobicity can be shortened.
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| Substances | Elution Volume (mL) | |
|---|---|---|
| Eluent (1) | Eluent (2) | |
| Oxalic acid | 9.28 | 9.62 |
| Maleic acid | 9.91 | 10.51 |
| α-Ketoglutaric acid | 10.39 | 10.87 |
| Citric acid | 10.99 | 11.71 |
| Isocitric acid | 11.05 | 11.88 |
| 2-Ketogluconic acid | 11.18 | 11.52 |
| Glucuronic acid | 11.29 | 11.71 |
| Oxaloacetic acid | 11.40 | 11.77 |
| Pyruvic acid | 11.42 | 11.76 |
| Phosphoric acid | 11.43 | 11.27 |
| Tartaric acid | 11.53 | 12.14 |
| trans-Aconitic acid | 11.57 | 13.11 |
| Galacturonic acid | 11.90 | 12.41 |
| Malonic acid | 12.10 | 12.96 |
| Gluconic acid | 12.24 | 12.78 |
| Malic acid | 12.37 | 13.37 |
| Glyoxylic acid | 12.70 | 13.26 |
| α-Hydroxyglutaric acid | 12.71 | 14.02 |
| Ascorbic acid | 12.71 | 13.88 |
| Succinic acid | 13.77 | 15.79 |
| Itaconic acid | 14.08 | 16.80 |
| Fumaric acid | 14.14 | 17.16 |
| Uric acid | 14.99 | 18.31 |
| Glycolic acid | 15.12 | 16.26 |
| Adipic acid | 15.16 | 20.17 |
| Lactic acid | 15.23 | 16.67 |
| 2-Hydroxyisobutyric acid | 15.26 | 17.05 |
| Mesaconic acid | 15.42 | 20.47 |
| β-Hydroxypropionic acid | 15.61 | 17.18 |
| Levulinic acid | 16.44 | 20.27 |
| Formic acid | 16.59 | 17.86 |
| Acetic acid | 17.32 | 19.41 |
| Pyroglutamic acid | 18.02 | 20.99 |
| Propionic acid | 18.82 | 22.54 |
| Isobutyric acid | 20.13 | 25.59 |
| n-Butyric acid | 20.87 | 27.61 |
| Isovaleric acid | 22.16 | 32.28 |
| HCO3- | 23.36 | 24.50 |
| n-Valeric acid | 24.64 | 38.89 |
- Columns
- ：Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
- Eluent
- ：(1) ; 3 mM HClO4 aq./CH3CN=90/10
(2) ; 3 mM HClO4 aq. - Flow rate
- ：1.0 mL/min
- Column temp.
- ：50 ℃
Sample Name Index
- 2-Hydroxyisobutyric acid
- 2-Ketogluconic acid
- Acetic acid
- Adipic acid
- Ascorbic acid, Vitamin C
- Citric acid
- D-Glucuronic acid
- Formic acid
- Fumaric acid
- Galacturonic acid
- Gluconic acid
- Glycolic acid
- Glyoxylic acid
- Hydrogencarbonate, HCO3-
- Isobutyric acid
- Isocitric acid
- Isovaleric acid
- Itaconic acid
- Lactic acid
- Levulinic acid
- Maleic acid
- Malic acid
- Malonic acid
- Mesaconic acid
- Oxalic acid
- Oxaloacetic acid
- Phosphoric acid
- Propionic acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
- Tartaric acid
- Uric acid
- n-Butyric acid
- n-Valeric acid
- trans-Aconitic acid
- α-Hydroxyglutaric acid
- α-Ketoglutaric acid
- β-Hydroxypropionic acid, Hydracrylic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Influence of Acidic Eluent Selection on Organic Acid Analysis (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Beer (KC-811)
- Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Reverse Phase Analysis of Gluconic Acids (DE-613)
- Analysis of Organic Acids in Sake (Japanese Rice Wine) (KC-811)
- Analysis of Organic Acids in White Wine (KC-811)
- Analysis of Oxalic Acid Using Ion Exclusion Mode (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Soy Sauces (KC-811)
- Organic Acids in Vinegar (KC-811)
- Separation of Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Analysis of Organic Acids in Soybean Paste (KC-811)
- Analysis of Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Elution Volume of Organic Acids (NI-424)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)
- Organic Acid Determination Test for Glycerin Fatty Acid Esters According to Japan’s Specifications and Standards for Food Additives (SH1011) (SH1011)