Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)

Organic acid standards were analyzed using RSpak KC-811. The elution time of organic acids which have long carbon chain such as butyric acid or valeric acid can be shortened adding cyclodextrin in the eluent.

Sample: 5 mM each, 20 μL

  1. 1.Fumaric acid    10.Isovaleric acid
  2. 2.Succinic acid
  3. 3.Lactic acid
  4. 4.Formic acid
  5. 5.Acetic acid
  6. 6.Propionic acid
  7. 7.n-Butyric acid
  8. 8.Isobutyric acid
  9. 9.n-Valeric acid
Column
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent
2 mM HClO4 + 4 mM α-Cyclodextrin (pH3.0)
Flow rate
(Eluent); 1.0 mL/min, (Reagent); 0.5 mL/min
Detector
VIS (430 nm) *Post column method
Column temp.
47 ℃

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