Organic acid standards were analyzed using RSpak KC-811. The elution time of organic acids which have long carbon chain such as butyric acid or valeric acid can be shortened adding cyclodextrin in the eluent.

Sample : 5 mM each , 20 μL
1. Fumaric acid
2. Succinic acid
3. Lactic acid
4. Formic acid
5. Acetic acid
6. Propionic acid
7. n-Butyric acid
8. Isobutyric acid
9. n-Valeric acid
10. Isovaleric acid

Sample : 5 mM each , 20 μL
1. Fumaric acid
2. Succinic acid
3. Lactic acid
4. Formic acid
5. Acetic acid
6. Propionic acid
7. n-Butyric acid
8. Isobutyric acid
9. n-Valeric acid
10. Isovaleric acid
Columns : Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2 Eluent : 2 mM HClO4 + 4 mM α-cyclodextrin (pH3.0) Flow rate : (Eluent); 1.0 mL/min, (Reagent); 0.5 mL/min Detector : VIS (430 nm) (Post column method) Column temp. : 47 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Beer (KC-811)
- Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Gluconic Acids (DE-613)
- Sake (Japanese Rice Wine) (KC-811)
- Organic Acids in White Wine (KC-811)
- Oxalic Acid (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Soy Source (KC-811)
- Organic Acids in Vinegar (KC-811)
- Succinic Acid and Fumaric Acid (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Organic Acids in Soybean Paste (KC-811)
- Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)