Organic acid standards were analyzed using RSpak KC-811. The elution time of organic acids which have long carbon chain such as butyric acid or valeric acid can be shortened adding cyclodextrin in the eluent.


Sample : 5 mM each , 20 μL
1. Fumaric acid
2. Succinic acid
3. Lactic acid
4. Formic acid
5. Acetic acid
6. Propionic acid
7. n-Butyric acid
8. Isobutyric acid
9. n-Valeric acid
10. Isovaleric acid

Columns      : Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent       : 2 mM HClO4 + 4 mM α-cyclodextrin (pH3.0)
Flow rate    : (Eluent); 1.0 mL/min, (Reagent); 0.5 mL/min 
Detector     : VIS (430 nm) (Post column method)
Column temp. : 47 °C