When water or 100 % buffer is used as the eluent, the column life of ODS columns is usually shorten. Since the packing material of RSpak DE-413 is chemically stable, long column life and stable analysis results can be obtained even when Water or 100 % buffer was used as the eluent.


Sample : 5 μL
1. Glyoxylic acid, 1.78 mg/mL
2. Tartaric acid, 1.95 mg/mL
3. Malic acid, 2.06 mg/mL
4. Lactic acid, 2 μL/mL
5. Malonic acid, 1.95 mg/mL
6. Acetic acid, 2 μL/mL
7. Succinic acid, 2.05 mg/mL
8. Levulinic acid, 1.95 mg/mL
9. Propionic acid, 2 μL/mL

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : 10 mM H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 50 °C