Acetic acid is a functional food component which is effective in reducing visceral fat, lowering blood pressure, and relieving fatigue. In this application, acetic acid in vinegar drinks was analyzed using RSpak KC-811. The vinegar dink can be analyzed after a simple dilution. A good linear calibration was obtained for the concentration range of 1 to 1,000 mg/L. Generally, a highly selective post-column method is required for the analysis of organic acids in foods, which contain many components. However, for the analysis of vinegar drinks, a UV detector was effective for detecting the target, acetic acid, without the influence of other components.

(Sample Preparation)
1) Dilute the commercial vinegar drink 1+4 with water.
2) Filer using a 0.45-µm membrane filter and use it as an injection sample.


Sample : 20 µL
1. Acetic acid 1000 mg/L

Columns      : Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent       : 3 mM HClO4 aq.
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 50 °C