Citric Acid in Phosphoric Acid (DE-613 + KC-811)

Citric acid in phosphoric acid was analyzed using RSpak DE-613, a polymer-based reversed chromatography column and RSpak KC-811, an organic acids analysis column.

Sample: 100 µL

  1. 1.Phosphoric acid 5 mM
  2. 2.Citric acid 0.025 mM
  3. 3.Malic acid 0.025 mM
Column
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + DE-613 (6.0 mm I.D. x 150 mm) x 2 + KC-811 (8.0 mm I.D. x 300 mm) x 3
Eluent
2 mM HClO4 aq.
Flow rate
(Eluent); 1.0 mL/min, (Reagent); 0.5 mL/min
Detector
VIS (430 nm) *Post column method
Column temp.
50 ℃

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