As the pH of the eluent and the column temperature affect the dissociation of organic acids, they are important for the adjustment of separation when RSpak KC-811 is used.
Substance | Elution Volume (mL) | |||
---|---|---|---|---|
1 mM HClO4 aq. | 2 mM HClO4 aq. | 3 mM HClO4 aq. | 5 mM HClO4 aq. | |
Oxalic acid |
9.27
|
9.41
|
9.62
|
9.72
|
Maleic acid |
9.70
|
10.15
|
10.51
|
11.12
|
α-Ketoglutaric acid |
9.96
|
10.44
|
10.87
|
11.37
|
Oxaloacetic acid |
10.48
|
11.22
|
11.77
|
12.50
|
Pyruvic acid |
10.51
|
11.24
|
11.76
|
12.48
|
2-Ketogluconic acid |
10.59
|
11.13
|
11.52
|
11.73
|
Phosphoric acid |
10.61
|
10.95
|
11.27
|
11.58
|
Citric acid |
10.84
|
11.32
|
11.71
|
11.81
|
Tartaric acid |
11.06
|
11.66
|
12.14
|
12.31
|
Isocitric acid |
11.10
|
11.54
|
11.88
|
11.89
|
Glucuronic acid |
11.16
|
11.44
|
11.71
|
11.76
|
trans-Aconitic acid |
11.47
|
12.43
|
13.11
|
13.48
|
Malonic acid |
11.54
|
12.36
|
12.96
|
13.40
|
Galacturonic acid |
11.83
|
12.12
|
12.41
|
12.39
|
Gluconic acid |
12.29
|
12.53
|
12.78
|
12.48
|
Glyoxylic acid |
12.35
|
12.86
|
13.26
|
13.86
|
Malic acid |
12.50
|
12.99
|
13.37
|
13.42
|
α-Hydroxyglutaric acid |
13.47
|
13.91
|
14.02
|
14.15
|
Ascorbic acid |
13.53
|
13.69
|
13.88
|
13.80
|
Fumaric acid |
14.47
|
16.09
|
17.16
|
17.75
|
Succinic acid |
15.30
|
15.52
|
15.79
|
15.69
|
Glycolic acid |
15.60
|
15.92
|
16.26
|
16.22
|
Itaconic acid |
15.92
|
16.42
|
16.80
|
16.79
|
Lactic acid |
16.02
|
16.39
|
16.67
|
16.68
|
2-Hydroxyisobutyric acid |
16.52
|
16.82
|
17.05
|
17.05
|
Adipic acid |
16.65
|
19.84
|
20.17
|
20.01
|
β-Hydroxypropionic acid |
16.94
|
17.04
|
17.18
|
17.12
|
Formic acid |
17.18
|
17.55
|
17.86
|
17.92
|
Mesaconic acid |
17.20
|
19.20
|
20.47
|
21.06
|
Uric acid |
17.89
|
17.93
|
18.31
|
18.00
|
Pyroglutamic acid |
18.59
|
20.25
|
20.99
|
21.65
|
Acetic acid |
19.19
|
19.29
|
19.41
|
19.36
|
Levulinic acid |
20.01
|
20.19
|
20.27
|
20.23
|
Propionic acid |
22.33
|
22.43
|
22.54
|
22.49
|
HCO3- |
24.72
|
24.72
|
24.50
|
24.68
|
Isobutyric acid |
25.35
|
25.47
|
25.59
|
25.55
|
n-Butyric acid |
27.32
|
27.46
|
27.61
|
27.57
|
Isovaleric acid |
31.89
|
32.07
|
32.28
|
32.20
|
n-Valeric acid |
38.36
|
38.57
|
38.89
|
38.75
|
Columns : Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2 Eluent : HClO4 aq. Flow rate : 1.0 mL/min Column temp. : 50 °C
Sample Name Index
- 2-Hydroxyisobutyric acid
- 2-Ketogluconic acid
- Acetic acid
- Adipic acid
- Ascorbic acid, Vitamin C
- Citric acid
- D-Glucuronic acid
- Formic acid
- Fumaric acid
- Galacturonic acid
- Gluconic acid
- Glycolic acid
- Glyoxylic acid
- Hydrogencarbonate, HCO3-
- Isobutyric acid
- Isocitric acid
- Isovaleric acid
- Itaconic acid
- Lactic acid
- Levulinic acid
- Maleic acid
- Malic acid
- Malonic acid
- Mesaconic acid
- Oxalic acid
- Oxaloacetic acid
- Phosphoric acid
- Propionic acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
- Tartaric acid
- Uric acid
- n-Butyric acid
- n-Valeric acid
- trans-Aconitic acid
- α-Hydroxyglutaric acid
- α-Ketoglutaric acid
- β-Hydroxypropionic acid, Hydracrylic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Beer (KC-811)
- Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Gluconic Acids (DE-613)
- Sake (Japanese Rice Wine) (KC-811)
- Organic Acids in White Wine (KC-811)
- Oxalic Acid (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Soy Source (KC-811)
- Organic Acids in Vinegar (KC-811)
- Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Organic Acids in Soybean Paste (KC-811)
- Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)