As the pH of the eluent and the column temperature affect the dissociation of organic acids, they are important for the adjustment of separation when RSpak KC-811 is used.
Substances | Elution Volume (mL) | |||
---|---|---|---|---|
Eluent (1) | Eluent (2) | Eluent (3) | Eluent (4) | |
Oxalic acid | 9.27 | 9.41 | 9.62 | 9.72 |
Maleic acid | 9.70 | 10.15 | 10.51 | 11.12 |
α-Ketoglutaric acid | 9.96 | 10.44 | 10.87 | 11.37 |
Oxaloacetic acid | 10.48 | 11.22 | 11.77 | 12.50 |
Pyruvic acid | 10.51 | 11.24 | 11.76 | 12.48 |
2-Ketogluconic acid | 10.59 | 11.13 | 11.52 | 11.73 |
Phosphoric acid | 10.61 | 10.95 | 11.27 | 11.58 |
Citric acid | 10.84 | 11.32 | 11.71 | 11.81 |
Tartaric acid | 11.06 | 11.66 | 12.14 | 12.31 |
Isocitric acid | 11.10 | 11.54 | 11.88 | 11.89 |
Glucuronic acid | 11.16 | 11.44 | 11.71 | 11.76 |
trans-Aconitic acid | 11.47 | 12.43 | 13.11 | 13.48 |
Malonic acid | 11.54 | 12.36 | 12.96 | 13.40 |
Galacturonic acid | 11.83 | 12.12 | 12.41 | 12.39 |
Gluconic acid | 12.29 | 12.53 | 12.78 | 12.48 |
Glyoxylic acid | 12.35 | 12.86 | 13.26 | 13.86 |
Malic acid | 12.50 | 12.99 | 13.37 | 13.42 |
α-Hydroxyglutaric acid | 13.47 | 13.91 | 14.02 | 14.15 |
Ascorbic acid | 13.53 | 13.69 | 13.88 | 13.80 |
Fumaric acid | 14.47 | 16.09 | 17.16 | 17.75 |
Succinic acid | 15.30 | 15.52 | 15.79 | 15.69 |
Glycolic acid | 15.60 | 15.92 | 16.26 | 16.22 |
Itaconic acid | 15.92 | 16.42 | 16.80 | 16.79 |
Lactic acid | 16.02 | 16.39 | 16.67 | 16.68 |
2-Hydroxyisobutyric acid | 16.52 | 16.82 | 17.05 | 17.05 |
Adipic acid | 16.65 | 19.84 | 20.17 | 20.01 |
β-Hydroxypropionic acid | 16.94 | 17.04 | 17.18 | 17.12 |
Formic acid | 17.18 | 17.55 | 17.86 | 17.92 |
Mesaconic acid | 17.20 | 19.20 | 20.47 | 21.06 |
Uric acid | 17.89 | 17.93 | 18.31 | 18.00 |
Pyroglutamic acid | 18.59 | 20.25 | 20.99 | 21.65 |
Acetic acid | 19.19 | 19.29 | 19.41 | 19.36 |
Levulinic acid | 20.01 | 20.19 | 20.27 | 20.23 |
Propionic acid | 22.33 | 22.43 | 22.54 | 22.49 |
HCO3- | 24.72 | 24.72 | 24.50 | 24.68 |
Isobutyric acid | 25.35 | 25.47 | 25.59 | 25.55 |
n-Butyric acid | 27.32 | 27.46 | 27.61 | 27.57 |
Isovaleric acid | 31.89 | 32.07 | 32.28 | 32.20 |
n-Valeric acid | 38.36 | 38.57 | 38.89 | 38.75 |
- Columns
- :Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) x 2
- Eluent
- :(1) ; 1 mM HClO4 aq.
(2) ; 2 mM HClO4 aq.
(3) ; 3 mM HClO4 aq.
(4) ; 5 mM HClO4 aq. - Flow rate
- :1.0 mL/min
- Column temp.
- :50 ℃
Sample Name Index
- 2-Hydroxyisobutyric acid
- 2-Ketogluconic acid
- Acetic acid
- Adipic acid
- Ascorbic acid, Vitamin C
- Citric acid
- D-Glucuronic acid
- Formic acid
- Fumaric acid
- Galacturonic acid
- Gluconic acid
- Glycolic acid
- Glyoxylic acid
- Hydrogencarbonate, HCO3-
- Isobutyric acid
- Isocitric acid
- Isovaleric acid
- Itaconic acid
- Lactic acid
- Levulinic acid
- Maleic acid
- Malic acid
- Malonic acid
- Mesaconic acid
- Oxalic acid
- Oxaloacetic acid
- Phosphoric acid
- Propionic acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
- Tartaric acid
- Uric acid
- n-Butyric acid
- n-Valeric acid
- trans-Aconitic acid
- α-Hydroxyglutaric acid
- α-Ketoglutaric acid
- β-Hydroxypropionic acid, Hydracrylic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Influence of Acidic Eluent Selection on Organic Acid Analysis (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Beer (KC-811)
- Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Reverse Phase Analysis of Gluconic Acids (DE-613)
- Analysis of Organic Acids in Sake (Japanese Rice Wine) (KC-811)
- Analysis of Organic Acids in White Wine (KC-811)
- Analysis of Oxalic Acid Using Ion Exclusion Mode (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Soy Sauces (KC-811)
- Organic Acids in Vinegar (KC-811)
- Separation of Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Analysis of Organic Acids in Soybean Paste (KC-811)
- Analysis of Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Elution Volume of Organic Acids (NI-424)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)
- Organic Acid Determination Test for Glycerin Fatty Acid Esters According to Japan’s Specifications and Standards for Food Additives (SH1011) (SH1011)