Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)

Connection of the reversed phase column (RSpak DE-613) to RSpak KC-811 makes it possible to separate phosphoric acid, which is eluted earlier, from other organic acids.

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Substances Elution Volume (mL)
DE-613 KC-G 8B + KC811 x 2 KC-G 8B + DE-613 + KC-811 x 2
Oxalic acid   3.0 10.5 13.5
Maleic acid   4.2 11.4 15.6
Phosphoric acid   3.0 11.4 14.4
α-Ketoglutaric acid   3.2 11.5 14.7
Pyruvic acid   4.0 12.3 16.3
Citric acid   5.0 12.3 17.3
D-Glucuronic acid   3.3 12.3 15.6
Isocitric acid   5.0 12.6 17.6
Tartaric acid   3.8 13.0 16.8
Gluconic acid   3.4 13.5 16.9
Malonic acid   5.3 13.6 18.9
trans-Aconitic acid 15.7 13.7 29.4
Glyoxylic acid   3.7 14.0 17.7
Malic acid   5.1 14.1 19.2
Succinic acid   8.6 16.8 25.2
Glycolic acid   4.3 17.0 21.3
Lactic acid   5.5 17.5 23.0
Itaconic acid 15.7 17.6 33.3
Fumaric acid 16.9 17.8 34.7
β-Hydroxypropionic acid   5.0 18.3 23.3
Formic acid   4.7 18.7 23.4
Uric acid   6.9 19.4 26.3
Acetic acid 6.3 20.4 26.7
Mesaconic acid 43.4 20.7 64.1
Adipic acid 30.2 21.1 51.3
Levulinic acid 11.5 21.2 32.7
L-Pyroglutamic acid   5.5 21.4 26.9
Propionic acid 12.8 23.9 36.7
HCO3-   7.8 25.7 33.5
Isobutyric acid 32.3 26.5 58.8
n-Butyric acid 32.6 28.5 61.1
Isovaleric acid Not eluted 32.9 Not eluted
n-Valeric acid Not eluted 39.7 Not eluted
Columns
Shodex RSpak DE-613 (6.0 mm I.D. x 150 mm)
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + DE-613 (6.0 mm I.D. x 150 mm) + KC-811 (8.0 mm I.D. x 300mm) x 2
Eluent
2 mM HClO4 aq.
Column temp.
50 ℃

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