Connection of the reversed phase column (RSpak DE-613) to RSpak KC-811 makes it possible to separate phosphoric acid, which is eluted earlier, from other organic acids.
Scroll
| Substances | Elution Volume (mL) | ||
|---|---|---|---|
| DE-613 | KC-G 8B + KC811 x 2 | KC-G 8B + DE-613 + KC-811 x 2 | |
| Oxalic acid | 3.0 | 10.5 | 13.5 |
| Maleic acid | 4.2 | 11.4 | 15.6 |
| Phosphoric acid | 3.0 | 11.4 | 14.4 |
| α-Ketoglutaric acid | 3.2 | 11.5 | 14.7 |
| Pyruvic acid | 4.0 | 12.3 | 16.3 |
| Citric acid | 5.0 | 12.3 | 17.3 |
| D-Glucuronic acid | 3.3 | 12.3 | 15.6 |
| Isocitric acid | 5.0 | 12.6 | 17.6 |
| Tartaric acid | 3.8 | 13.0 | 16.8 |
| Gluconic acid | 3.4 | 13.5 | 16.9 |
| Malonic acid | 5.3 | 13.6 | 18.9 |
| trans-Aconitic acid | 15.7 | 13.7 | 29.4 |
| Glyoxylic acid | 3.7 | 14.0 | 17.7 |
| Malic acid | 5.1 | 14.1 | 19.2 |
| Succinic acid | 8.6 | 16.8 | 25.2 |
| Glycolic acid | 4.3 | 17.0 | 21.3 |
| Lactic acid | 5.5 | 17.5 | 23.0 |
| Itaconic acid | 15.7 | 17.6 | 33.3 |
| Fumaric acid | 16.9 | 17.8 | 34.7 |
| β-Hydroxypropionic acid | 5.0 | 18.3 | 23.3 |
| Formic acid | 4.7 | 18.7 | 23.4 |
| Uric acid | 6.9 | 19.4 | 26.3 |
| Acetic acid | 6.3 | 20.4 | 26.7 |
| Mesaconic acid | 43.4 | 20.7 | 64.1 |
| Adipic acid | 30.2 | 21.1 | 51.3 |
| Levulinic acid | 11.5 | 21.2 | 32.7 |
| L-Pyroglutamic acid | 5.5 | 21.4 | 26.9 |
| Propionic acid | 12.8 | 23.9 | 36.7 |
| HCO3- | 7.8 | 25.7 | 33.5 |
| Isobutyric acid | 32.3 | 26.5 | 58.8 |
| n-Butyric acid | 32.6 | 28.5 | 61.1 |
| Isovaleric acid | Not eluted | 32.9 | Not eluted |
| n-Valeric acid | Not eluted | 39.7 | Not eluted |
- Columns
- ：Shodex RSpak DE-613 (6.0 mm I.D. x 150 mm)
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + DE-613 (6.0 mm I.D. x 150 mm) + KC-811 (8.0 mm I.D. x 300mm) x 2 - Eluent
- ：2 mM HClO4 aq.
- Column temp.
- ：50 ℃
Sample Name Index
- Acetic acid
- Adipic acid
- Citric acid
- D-Glucuronic acid
- Formic acid
- Fumaric acid
- Gluconic acid
- Glycolic acid
- Glyoxylic acid
- Hydrogencarbonate, HCO3-
- Isobutyric acid
- Isocitric acid
- Isovaleric acid
- Itaconic acid
- Lactic acid
- Levulinic acid
- Maleic acid
- Malic acid
- Malonic acid
- Mesaconic acid
- Oxalic acid
- Phosphoric acid
- Propionic acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
- Tartaric acid
- Uric acid
- n-Butyric acid
- n-Valeric acid
- trans-Aconitic acid
- α-Ketoglutaric acid
- β-Hydroxypropionic acid, Hydracrylic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Influence of Acidic Eluent Selection on Organic Acid Analysis (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Beer (KC-811)
- Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Reverse Phase Analysis of Gluconic Acids (DE-613)
- Analysis of Organic Acids in Sake (Japanese Rice Wine) (KC-811)
- Analysis of Organic Acids in White Wine (KC-811)
- Analysis of Oxalic Acid Using Ion Exclusion Mode (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Soy Sauces (KC-811)
- Organic Acids in Vinegar (KC-811)
- Separation of Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Analysis of Organic Acids in Soybean Paste (KC-811)
- Analysis of Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Elution Volume of Organic Acids (NI-424)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)
- Organic Acid Determination Test for Glycerin Fatty Acid Esters According to Japan’s Specifications and Standards for Food Additives (SH1011) (SH1011)