LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)

Pipecolic acid or piperidine-2-carboxlyic acid was analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Pipecolic acid has been proposed as a diagnostic indicator of pyridoxine-dependent epilepsy. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for the high sensitivity analysis by LC/MS.
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Sample : 5 μL
L-Pipecolic acid 10 ng/mL
(in H2O/CH3CN=25/75)

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : 20 mM HCOONH4 aq./CH3CN=20/80
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 30 °C

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