Pipecolic acid or piperidine-2-carboxlyic acid was analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Pipecolic acid has been proposed as a diagnostic indicator of pyridoxine-dependent epilepsy. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for the high sensitivity analysis by LC/MS.
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Sample : 5 μL
L-Pipecolic acid 10 ng/mL
(in H2O/CH3CN=25/75)
Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm) Eluent : 20 mM HCOONH4 aq./CH3CN=20/80 Flow rate : 0.2 mL/min Detector : ESI-MS (SIM Positive) Column temp. : 30 °C
Sample Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Beer (KC-811)
- Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Reverse Phase Analysis of Gluconic Acids (DE-613)
- Analysis of Organic Acids in Sake (Japanese Rice Wine) (KC-811)
- Analysis of Organic Acids in White Wine (KC-811)
- Analysis of Oxalic Acid Using Ion Exclusion Mode (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Soy Sauces (KC-811)
- Organic Acids in Vinegar (KC-811)
- Separation of Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Analysis of Organic Acids in Soybean Paste (KC-811)
- Analysis of Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Organic Acids (KC-811)
- Elution Volume of Organic Acids (NI-424)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)
- Organic Acid Determination Test for Glycerin Fatty Acid Esters According to Japan’s Specifications and Standards for Food Additives (SH1011) (SH1011)