Organic acids in beer are analyzed with 4.8 mM perchloric acid as an eluent using RSpak KC-811. When four KC-811 columns are connected in line, phosphoric acid, citric acid and pyruvic acid can be separated from each other completely.
Sample: 100 μL
Beer
- 1.Phosphoric acid etc.
- 2.Citric acid
- 3.Pyruvic acid
- 4.Gluconic acid
- 5.Malic acid
- 6.Succinic acid
- 7.Lactic acid
- 8.Fumaric acid
- 9.Acetic acid
- 10.Pyroglutamic acid
- 11.Carbonic acid
- Column
- ：(A) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
(B) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 m) x 3
(C) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 4 - Eluent
- ：4.8 mM HClO4 aq.
- Flow rate
- ：(Eluent); 1.0 mL/min, (Reagent); 1.1 mL/min
- Detector
- ：VIS (430 nm) *Post column method
- Column temp.
- ：63 ℃
Sample Name Index
- Acetic acid
- Beer
- Carbonate, CO32-
- Citric acid
- Fumaric acid
- Gluconic acid
- Hydrogencarbonate, HCO3-
- Lactic acid
- Malic acid
- Phosphoric acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Reverse Phase Analysis of Gluconic Acids (DE-613)
- Analysis of Organic Acids in Sake (Japanese Rice Wine) (KC-811)
- Analysis of Organic Acids in White Wine (KC-811)
- Analysis of Oxalic Acid Using Ion Exclusion Mode (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Soy Sauces (KC-811)
- Organic Acids in Vinegar (KC-811)
- Separation of Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Analysis of Organic Acids in Soybean Paste (KC-811)
- Analysis of Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Elution Volume of Organic Acids (NI-424)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)
- Organic Acid Determination Test for Glycerin Fatty Acid Esters According to Japan’s Specifications and Standards for Food Additives (SH1011) (SH1011)