Organic acids in beer are analyzed with 4.8 mM perchloric acid as an eluent using RSpak KC-811. When four KC-811 columns are connected in line, phosphoric acid, citric acid and pyruvic acid can be separated from each other completely.
Please refer to the right figure.
Sample : Beer, 100 μL
1. Phosphoric acid etc., 2. Citric acid, 3. Pyruvic acid, 4. Gluconic acid, 5. Malic acid
6. Succinic acid, 7. Lactic acid, 8. Fumaric acid, 9. Acetic acid, 10. Pyroglutamic acid, 11. Carbonic acid
Columns : (A) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2 (B) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 3 (C) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 4 Eluent : 4.8 mM HClO4 aq. Flow rate : (Eluent); 1.0 mL/min, (Reagent); 1.1 mL/min Detector : VIS (430 nm) (Post column method) Column temp. : 63 °C
Sample Name Index
- Acetic acid
- Beer
- Carbonate, CO32-
- Citric acid
- Fumaric acid
- Gluconic acid
- Hydrogencarbonate, HCO3-
- Lactic acid
- Malic acid
- Phosphoric acid
- Pyroglutamic acid
- Pyruvic acid
- Succinic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Aromatic Organic Acids (NN-814)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Gluconic Acids (DE-613)
- Sake (Japanese Rice Wine) (KC-811)
- Organic Acids in White Wine (KC-811)
- Oxalic Acid (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Organic Acids in Soy Source (KC-811)
- Organic Acids in Vinegar (KC-811)
- Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Organic Acids in Soybean Paste (KC-811)
- Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)