Organic acids in beer are analyzed with 4.8 mM perchloric acid as an eluent using RSpak KC-811. When four KC-811 columns are connected in line, phosphoric acid, citric acid and pyruvic acid can be separated from each other completely.
Please refer to the right figure.



Sample : Beer, 100 μL
1. Phosphoric acid etc., 2. Citric acid, 3. Pyruvic acid, 4. Gluconic acid, 5. Malic acid
6. Succinic acid, 7. Lactic acid, 8. Fumaric acid, 9. Acetic acid, 10. Pyroglutamic acid, 11. Carbonic acid

Columns      : (A) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) 
                   + KC-811 (8.0 mm I.D. x 300 mm) x 2
               (B) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm)
                   + KC-811 (8.0 mm I.D. x 300 mm) x 3
               (C) Shodex RSpak KC-G 6B (6.0 mm I.D. x 50 mm) 
                   + KC-811 (8.0 mm I.D. x 300 mm) x 4
Eluent       : 4.8 mM HClO4 aq.
Flow rate    : (Eluent); 1.0 mL/min, (Reagent); 1.1 mL/min 
Detector     : VIS (430 nm) (Post column method)
Column temp. : 63 °C