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Comparison of maltooligosaccharides separation

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Efficiencies of Aqueous/Organic (multi-solvent use) SEC columns, Asahipak GF-210 HQ and GF-310 HQ were compared for the separation of maltooligosaccharides. Compared to GF-310 HQ, peaks are separated better with GF-210 HQ. Tri- to hexa-saccharide which were all eluted together with GF-310 HQ were separated with GF-210 HQ, indicating its improved separation efficiency in the lower molecular weight range.


Sample : 0.1% each, 20μL
1. Maltohexaose
2. Maltopentaose
3. Maltotetraose
4. Maltotriose
5. Maltose
6. Glucose


Columns      : Shodex Asahipak GF=210 HQ x 2, GF-310 HQ x 2 (7.5mmI.D. x 300mm each)
Eluent       : H2O
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 50°C

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