Hydroxypropyl-β-cyclodextrin is added to pharmaceuticals, including vaccines, to improve the solubility in water, stabilizing, etc. It is also expected to be used as *adjuvant in recent years. In this application, trypsin inhibitor and hydroxypropyl-β-cyclodextrin were separated using OHpak SB-802.5 HQ. It is possible to measure and quantify hydroxypropyl-β-cyclodextrin without deproteinization by using a column for SEC. The calibration curve shows good linearity in the range from 0.05 to 10 mg/mL.
*Adjuvant: Generic term for factors that enhance the effect of vaccines
Sample : 1 mg/mL each, 50 µL
1. Trypsin Inhibitor
(MW: 20,000)
2. Hydroxypropyl-β-cyclodextrin
Columns : Shodex OHpak SB-802.5 HQ (8.0 mm I.D. x 300 mm) x 2 Eluent : **PBS(-)/CH3CN=80/20 Flow rate : 1.0 mL/min Detector : RI Column temp. : 30 °C **PBS(-) Phosphate buffered saline without calcium and magnesium
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Hydrolyzed Dextran (2) (KS-802)
- Hydrolyzed Dextran (3) (NH2P-50 4E)
- Hydrolyzed Dextran (5) (KS-802)
- Hydrolyzed Dextran (6) (VN-50 4D)
- Hydrolyzed Dextran (7) (VN-50 4D by Gradient Elution)
- HILIC Mode Separation of Dextran 1 Oligomers (VG-25 4D)
- Isomaltooligosaccharides (NH2P-50 4E)
- Isomaltooligosaccharides (2) (VG-50 4E)
- Maltooligosaccharides (NH2P-50)
- Maltooligosaccharides (2) (VG-50 4E)
- Comparison of maltooligosaccharides separation
- N-Acetylchitooligosaccharides (1) (NH2P-50 4E)
- N-Acetylchitooligosaccharides (2) (VN-50 4D)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (1) (NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (2) (Asahipak NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)
- Cyclodextrins (Comparison of GS-220 HQ and KS-802)
- Analysis of Cyclodextrins (NH2P-50 4E)
- Analysis of Cyclodextrins According to Japan's Specifications and Standards for Food Additives Method (KS-802)
- Short-Chain Amylose (1) (NH2P-50 10E)
- Short-Chain Amylose (2) (ODP-50 6D)
- Hydrolyzed Starch (2) (KS-802)
- Sweetener (KS-802)
- Effects of Flow Rate (SC1211)
- Fructooligosaccharide Syrup (NH2P-50 4E)
- Analysis of Fructooligosaccharide Components (KS-802 + KS-801)
- Analysis of Fructooligosaccharides in Accordance to the Food System for Specified Health Use (VG-50 4E)
- Starch Syrup
- Low-Molecular-Weight Water Soluble Dietary Fiber (GS-220 HQ, KS-802)
- Xylooligosaccharides (1) (KS-801)
- Xylooligosaccharides (2) (VN-50 4D)
- Xylooligosaccharides (3) (SH1011)
- Degradation Product of Cellulose with Cellulase (PcCel45A) Digestion
- Oligosaccharide in Soy Bean (KS-802 + KS-801)
- Analysis of Trisaccharides (1) (NH2P-40 3E)
- Analysis of Trisaccharides (2) (VG-50 4E)
- Galactooligosaccharides (1) (VG-50 4E)
- Galactooligosaccharides (2) (KS-801, NH2P-50 4E)
- Analysis of Maltodextrin (VN-50 4D)
- Analysis of Maltodextrin (2) (VG-25 4D)
- Separation of Kestose and Maltose (KS-802)