Hydroxypropyl-β-cyclodextrin is added to pharmaceuticals, including vaccines, to improve the solubility in water, stabilizing, etc. It is also expected to be used as ^*adjuvant in recent years. In this application, trypsin inhibitor and hydroxypropyl-β-cyclodextrin were separated using OHpak SB-802.5 HQ​. It is possible to measure and quantify hydroxypropyl-β-cyclodextrin without deproteinization by using a column for SEC. The calibration curve shows good linearity in the range from 0.05 to 10 mg/mL.
^*Adjuvant: Generic term for factors that enhance the effect of vaccines


Sample : 1 mg/mL each, 50 µL
1. Trypsin Inhibitor
(MW: 20,000)
2. Hydroxypropyl-β-cyclodextrin

Columns      : Shodex OHpak SB-802.5 HQ (8.0 mm I.D. x 300 mm) x 2
Eluent       : **PBS(-)/CH3CN=80/20
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 30 °C

**PBS(-) Phosphate buffered saline without calcium and magnesium