Xylooligosaccharides were analyzed using SUGAR SH1011, a column for saccharides and organic acids analysis, in accordance to the regulations in standardized FOSHU (Food for Specified Health Uses) in Japan. The content (%) of xylooligosaccharides and the content (%) of xylobiose in xylooligosaccharides are determined in the regulation. In this analysis method, all of peaks eluting faster than xylobiose and xylobiose are regarded as xylooligosaccharides.


Sample : 10 μL
5 % Xylooligosaccharides std.
1. Xylobiose
2. Glucose
3. Xylose

Column       : Shodex SUGAR SH1011 (8.0 mm I.D. x 300 mm)
Eluent       : 5 mM H2SO4 aq.
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : 65 °C