According to the Quantitative Method for “Fructooligosaccharides (2)” listed in the Japanese Ingredient Standards for Foods for Specified Health Uses (FOSHU), a column packed with aminopropyl-silylated modified silica gel is used. In this application, a standard fructooligosaccharide (powder) with the internal standard (glycerin) was analyzed using a silica-based amino column and HILICpak VG-50 4E, a polymer-based amino column in reference to the FOSHU analysis method. The FOSHU requires calculating the content (%) of fructooligosaccharides (the total of 1-kestose, nystose, and 1F-fructosylnystose). The quantification results using VG-50 4E showed that the fructooligosaccharide content in the sample was 96.2%, with the component ratio of 1-kestose : nystose : 1F-fructosylnystose being 39.3 : 53.0 : 7.7, confirming compliance with each component specification. Although similar results can be achieved with a silica-based amino column, VG-50 4E shows an advantage in reducing the analysis time to about one-third. Additionally, polymer-based amino columns offer greater durability compared to silica-based columns.

Sample: 10 µL each
20 mg/mL of Fructooligosaccharides and 10 mg/mL of Glycerin in H₂O

1. 1. 1.Glycerin (Internal standard)
   2. 2.1-Kestose (GF₂)
   3. 3.Nystose (GF₃)
   4. 4.Fructofranosylnystose (GF₄)