According to the standard of the food for specified health use (FOSHU), "Assay" for "Fructooligosaccharides (1)" should be carried out with a column filled with chemically modified aminopropyl silylated silica gel. In this application, a standard fructooligosaccharide (powder) with the internal standard (glycerin) was analyzed using a silica-based amino column and the HILICpak VG-50 4E, a polymer-based amino column. The FOSHU requires calculating the content (%) of fructooligosaccharides (the total of 1-kestose, nystose, and 1F-fructosylnystose). The quantification results using VG-50 4E showed that the fructooligosaccharide content in the sample was 96.2%, with the component ratio of 1-kestose: nystose: 1F-fructosylnystose being 39.3: 53.0: 7.7, confirming compliance with each component specification. Although similar results can be achieved with a silica-based amino column, using VG-50 4E reduces the analysis time to about one-third. Additionally, polymer-based amino columns offer greater durability compared to silica-based ones, making VG-50 4E suitable for this analysis.

Sample: 10 µL each
20 mg/mL of Fructooligosaccharides and 10 mg/mL of Glycerin in H₂O

1. 1. 1.Glycerin (Internal standard)
   2. 2.1-Kestose (GF₂)
   3. 3.Nystose (GF₃)
   4. 4.Fructofranosylnystose (GF₄)