Japanese Notification Eishin No. 47* (May 23, 1996, Ministry of Health, Labour and Welfare (MHLW)) “Analytical Methods for Nutritional Components, Following the Introduction of Nutrition Labeling Standards” includes “Appendix: Analytical Methods for Nutritional Components”. Under section 8. Dietary Fiber, (2) High-Performance Liquid Chromatography Method (Enzyme-HPLC Method) targets foods containing low-molecular-weight water-soluble dietary fibers that are difficult to analyze using the Prosky method. In the enzyme-HPLC method, the indigestible fraction of trisaccharides and higher (indigestible oligosaccharides + dietary fiber fraction) is separated from other fractions (monosaccharides, disaccharides, and sugar alcohol fraction), and the ratio of the indigestible fraction is determined. Two columns, either ligand-exchange or gel-filtration types, are connected in series. Asahipak GS-220 HQ is listed as an example of a gel-filtration column. In this study, we used Asahipak GS-220 HQ to analyze low-molecular-weight water-soluble dietary fibers. For comparison, we also analyzed using a ligand-exchange column, SUGAR KS-802. GS-220 HQ enables easy separation of the indigestible fraction from other fractions in size-exclusion mode: in the chromatogram, the indigestible fraction elutes before the indicated arrow, while the other fractions elute after. On the other hand, KS-802 operates in both size-exclusion and ligand-exchange modes, which provides excellent separation among monosaccharides and disaccharides; however, its complex separation behavior makes it difficult to simply divide into indigestible and other fractions. Therefore, GS-220 HQ is highly effective for quantitative analysis of low-molecular-weight water-soluble dietary fibers.

*The version at the time of the application acquisition.

Sample: 20 µL each
Low molecular weight water soluble dietary fiber

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