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Xylooligosaccharides (2) (VN-50 4D)

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Xylooligosaccharides were separated using HILICpak VN-50 4D, a polymer-based HILIC column, with evaporative light scattering detector (ELSD). Oligosaccharides up to 13-mer could be confirmed in this sample.


Sample : 10μL
Xylooligosaccharides 5mg/mL (in 50% CH3CN)
1. Xylose
2. Xylobiose
3. Xylotriose
4. Xylotetraose
5. Xylopentaose
7. Xylohexaose
8. Xyloheptaose
9. Xylooctaose
10. Xylodecaose
11. Xyloundecaose
12. Xylododecaose
13. Xylotridecaose


Column       : Shodex HILICpak VN-50 4D (4.6mm I.D. x 150mm)
Eluent       : (A); 0.1% NH3 aq. (B); CH3CN
               Linear gradient ; (B%) 80% to 50% (0 to 20min)
Flow rate    : 1.0mL/min
Detector     : ELS
Column temp. : 40°C

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