N-Acetylchitooligosaccharides were analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis).


Sample : 20 μL
2 % Mixture of N-Acetylchitooligosaccharides
(Seikagaku Co. code:800104 Chitooligosaccharides Mixture)

1. N-Acetyl-D-glucosamine
2. di-N-Acetylchitobiose
3. tri-N-Acetylchitotriose
4. tetra-N-Acetylchitotetraose
5. penta-N-Acetylchitopentaose
6. hexa-N-Acetylchitohexaose

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 25 °C