Galactooligosaccharides which are the saccharides of dimer to hexamer composed mainly of galactose, are abundant in breast milk. Galactooligosaccharides are known to promote proliferation of intestinal Bifidobacterium. In this application, galactooligosaccharides are analyzed using HILICpak VG-50 4E, a column for saccharides analysis. It was confirmed that several oligosaccharides including the 4'-galactosyl lactose, a typical component of the galactooligosaccharide, were eluted.


Sample : 10 μL
Galactooligosaccharides 2.5 mg/mL
1. Lactose
2. 4'-Galactosyllactose

Column       : Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C