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Analysis of Maltodextrin (VN-50 4D)

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A unit called dextrose equivalent (DE) is sometimes used to indicate the degree of polymerization (DP) for starches. DE=0 indicates the state of starch, while when the starch is hydrolyzed and its molecular weight decreases, the DE value increases.
Titration is one way to measure DE value, but it is not a precise method to determine their fine differences.
In this application, we used a HILICpak VN-50 4D to separate and analyze various maltodextrins with different DE values. The chromatograms provide the information on their DE scale; as it shows that the samples with smaller DE values have maltodextrins with higher DP.   




Sample: 10 μL, 0.33 % (w/v) each
Maltodextrin DE 16.5-19.5
Maltodextrin DE 13-17
Maltodextrin DE 4-7


Column       : Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : (A); H2O (B); CH3CN
               Linear gradient ; (B %) 70 % to 50 % (0 to 20 min)
Flow rate    : 1.0 mL/min
Detector     : ELS
Column temp. : 40 °C

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