According to the Japanese Ingredient Standards for Foods for Specified Health Uses (FOSHU), “Galactooligosaccharides (1)” refers to galactooligosaccharides produced from lactose via the action of β‑galactosidase. In the Quantitative Method, galactooligosaccharides (disaccharides to hexasaccharides; DP2 – DP6) are quantified using a size‑exclusion ion‑exchange column packed with Na‑type sulfonated polystyrene gel. Lactose and 4’‑galactosyllactose in galactooligosaccharides are quantified using a normal‑phase column packed with polyamine‑type polymer gel. The galactooligosaccharide content is defined as the value obtained by subtracting the lactose content from the total content of galactooligosaccharides (DP2 – DP6). In this application, a galactooligosaccharide standard was analyzed using SUGAR KS‑801, a column for sugar analysis, and Asahipak NH2P‑50 4E, a polymer‑based amino column. In the KS‑801 chromatogram, the peaks to the left of the red dashed line correspond to DP2 and higher (two saccharides or more).
Note: The inner diameter (I.D.) of KS‑801 is 8.0 mm, which differs from the 6.0 mm I.D. described in the FOSHU.

Sample: 10 µL
2.5% of Galactooligosaccharides in H₂O

1. 1. 1.4'-Galactosyllactose
   2. 2.Lactose