Hydrolyzed Dextran (3) (NH2P-50 4E)

Asahipak NH2P-50 4E is used for the separation of hydorolyzed dextran, and two methods, isocratic elution and gradient elution are compared. Using gradient elution, better separation can be obtained. Until recently, gradient elution could not be used for the separation of sugars because there was no detector to be used. ELSD (evaporative light scattering detector) solved this problem and enables the separtion of sugars using gradient elution.

Sample:
Hydrolyzed Dextran 0.5 %

  1. (A)5 µL
  2. (B)20 µL
Column
：Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
：(A); CH3CN/H2O=50/50
(B); Linear gradient;
0 min to 7 min, CH3CN/H2O=57/43 to 50/50
7 min to 16 min, CH3CN/H2O=50/50
Flow rate
：1.0 mL/min
Detector
：ELSD
Column temp.
：Room temp.

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