Asahipak ODP-50, a polymer-based reversed phase column, with strong alkaline eluent to prevent anomer separation, oligosaccharides can be separated at room temperature by reversed phase mode. This is an example of one application in which ODP-50 6D enables the separation of oligosaccharides at room temperature with a strong alkaline eluent because polymer-based reversed phase column is resistant to high alkali.


Sample : Amylose

Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : NaOH aq. (pH10.75)
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 30 °C

K. Koizumi and T. Utamura, J. Chromatogr., 436 (1988) 328.