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Effects of Flow Rate (SC1211)

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A mixture of oligosaccharides, sugar alcohols and ethanol is separated using SUGAR SC1211 (a column for saccharides analysis) with the flow rate of 1.0mL/min and 0.5mL/min. The separation can be improved using lower flow rate.


Sample : 5μL
1. 0.5% Pullulan P-10
2. 1.0% Maltotriose
3. 1.0% Maltose
4. 1.0% Glucose
5. 1.0% Fructose
6. 1.0% Glycerol
7. 2.0% Ethanol
8. 1.0% Sorbitol


Columns      : Shodex SUGAR SC1211 (6.0mmI.D. x 250mm)
Eluent       : H2O
Flow rate    : (A); 1.0mL/min, (B); 0.5mL/min
Detector     : Shodex RI
Column temp. : 80°C

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