Effects of flow rate on the separation of SUGAR SC1211, a column for saccharides analysis, were studied. A mixture of oligosaccharides, sugar alcohols, and ethanol was separated at the flow rate of 1.0 mL/min and 0.5 mL/min. Improved separation was obtained with the lower flow rate.


Sample : 5 μL
1. Pullulan P-10 0.5 %
2. Maltotriose 1.0 %
3. Maltose 1.0 %
4. Glucose 1.0 %
5. Fructose 1.0 %
6. Glycerol 1.0 %
7. Ethanol 2.0 %
8. Sorbitol 1.0 %

Columns      : Shodex SUGAR SC1211 (6.0 mm I.D. x 250 mm)
Eluent       : H2O
Flow rate    : (A); 1.0 mL/min, (B); 0.5 mL/min
Detector     : RI
Column temp. : 80 °C