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Analysis of Cyclodextrins (NH2P-50 4E)


Three cyclodextrins were separated using Asahipak NH2P-50 4E, a polymer-based amino column. These conditions lead to a short elution time with greater resolution compared to the separation using an SEC column.

Sample : 20μL
250μg/mL each (in H2O/CH3CN=50/50)
1. α-Cyclodextrin
2. γ-Cyclodextrin
3. β-Cyclodextrin

Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : CH3CN/H2O=60/40
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 40°C

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