N-Acetylchitooligosaccharides (Chitin oligosaccharides) were separated using HILICpak VN-50 4D, a polymer-based HILIC column, with evaporative light scattering detector (ELSD). Oligosaccharides up to a heptamer could be analyzed in this sample.
Sample: 10 μL
N-Acetylchitooligosaccharides 5mg/mL (in 50 % CH3CN)
- 1.N-Acetyl-D-glucosamine
- 2.di-N-Acetylchitobiose
- 3.tri-N-Acetylchitotriose
- 4.tetra-N-Acetylchitotetraose
- 5.penta-N-Acetylchitopentaose
- 6.hexa-N-Acetylchitohexaose
- 7.hepta-N-Acetylchitohexaose
- Column
- ：Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
- Eluent
- ：(A); H2O (B); CH3CN
Linear gradient; (B %) 80 % to 65 % (0 to 10 min) - Flow rate
- ：1.0 mL/min
- Detector
- ：ELSD
- Column temp.
- ：60 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Hydrolyzed Dextran (2) (KS-802)
- Hydrolyzed Dextran (3) (NH2P-50 4E)
- Hydrolyzed Dextran (5) (KS-802)
- Hydrolyzed Dextran (6) (VN-50 4D)
- Hydrolyzed Dextran (7) (VN-50 4D by Gradient Elution)
- Isomaltooligosaccharides (NH2P-50 4E)
- Isomaltooligosaccharides (2) (VG-50 4E)
- Maltooligosaccharides (NH2P-50)
- Maltooligosaccharides (2) (VG-50 4E)
- Comparison of maltooligosaccharides separation
- N-Acetylchitooligosaccharides (1) (NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (1) (NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (2) (Asahipak NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)
- Cyclodextrins (Comparison of GS-220 HQ and KS-802)
- Analysis of Cyclodextrins (NH2P-50 4E)
- Analysis of Cyclodextrins According to Japan's Specifications and Standards for Food Additives Method (KS-802)
- Short-Chain Amylose (1) (NH2P-50 10E)
- Short-Chain Amylose (2) (ODP-50 6D)
- Hydrolyzed Starch (2) (KS-802)
- Sweetener (KS-802)
- Effects of Flow Rate (SC1211)
- Fructooligosaccharide Syrup (NH2P-50 4E)
- Analysis of Fructooligosaccharide Components (KS-802 + KS-801)
- Analysis of Fructooligosaccharides in Accordance to the Food System for Specified Health Use (VG-50 4E)
- Starch Syrup
- Low-Molecular-Weight Water Soluble Dietary Fiber (GS-220 HQ, KS-802)
- Xylooligosaccharides (1) (KS-801)
- Xylooligosaccharides (2) (VN-50 4D)
- Xylooligosaccharides (3) (SH1011)
- Degradation Product of Cellulose with Cellulase (PcCel45A) Digestion
- Oligosaccharide in Soy Bean (KS-802 + KS-801)
- Analysis of Trisaccharides (1) (NH2P-40 3E)
- Analysis of Trisaccharides (2) (VG-50 4E)
- Galactooligosaccharides (1) (VG-50 4E)
- Galactooligosaccharides (2) (KS-801, NH2P-50 4E)
- Hydroxypropyl-β-cyclodextrin (SB-802.5 HQ)
- Analysis of Maltodextrin (VN-50 4D)
- Separation of Kestose and Maltose (KS-802)