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Comparison of Separation using HK-404L with Conventional Device and Semi-micro Type Device (HK-404L)


The separation of polystyrene and the calculation result of molecular weight distribution of styrene butyl methacrylate copolymer using GPC HK-404L, an organic SEC (GPC) column for ultra-rapid analysis, were compared with a conventional device and a semi-micro type device. It was confirmed that the separation and the calculation result of molecular weight distribution are similar. HK-404L can be also used with the conventional device.

Sample : 5µL
1. Mp: 3,150,000 0.05%
2. Mp: 275,000 0.1%
3. Mp: 19,600 0.1%
4. Mp: 1,270 0.1%

Sample : 5µL
Styrene butyl methacrylate copolymer 0.1%(w/v)

*Peak heights are adjusted to make it easy to compare them.

Semi-micro typeConventional type
Injector Rheodyne 8125 Rheodyne 7725i
Tubing 0.1mm I.D. 0.25mm I.D.
RI cell volume 2.5μL 8μL
Columns      : Shodex GPC HK-404L (4.6mmI.D. x 150mm) x 2
Eluent       : THF              
Flow rate    : 1.0mL/min
Detector     : RI
Column temp. : 40°C

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