Comparison of Separation using HK-404L with Conventional Device and Semi-micro Type Device (HK-404L)

The separation of polystyrene and the calculation result of molecular weight distribution of styrene butyl methacrylate copolymer using GPC HK-404L, an organic SEC (GPC) column for ultra-rapid analysis, were compared with a conventional device and a semi-micro type device. It was confirmed that the separation and the calculation result of molecular weight distribution are similar. HK-404L can be also used with the conventional device.

Sample : 5 µL
1. Mp: 3,150,000 0.05 %
2. Mp: 275,000 0.1 %
3. Mp: 19,600 0.1 %
4. Mp: 1,270 0.1 %

Sample : 5 µL
Styrene butyl methacrylate copolymer 0.1 %(w/v)

*Peak heights are adjusted to make it easy to compare them.

Semi-micro typeConventional type
Injector Rheodyne 8125 Rheodyne 7725i
Tubing 0.1 mm I.D. 0.25 mm I.D.
RI cell volume 2.5 μL 8 μL
Columns      : Shodex GPC HK-404L (4.6 mm I.D. x 150 mm) x 2
Eluent       : THF              
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C

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