Effects of Injection Volume (KF-402HQ)

The effect of injection volume on theoretical plate numbers (TPN) is investigated for GPC KF-402HQ, an organic SEC semi-micro column. Injection volume of 5 µL or less is appropriate for KF-402HQ.

Sample : Dicyclohexyl phthalate

Column       : Shodex GPC KF-402HQ (4.6 mm I.D. x 250 mm each)
Eluent       : THF              
Flow rate    : 0.3 mL/min
Column temp. : 25 °C

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