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Effects of Injection Volume (KF-402HQ, KF-406LHQ)

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The effect of injection volume on theoretical plate numbers (TPN) is investigated for organic SEC semi-micro columns GPCKF-402HQ and KF-406LHQ. Injection volume of 5µL or less is appropriate for KF-402HQ and injection volume of 20µL or below is adequate for using 2 KF-406LHQ columns.


Sample : Dicyclohexyl phthalate


Columns      : Shodex GPC KF-402HQ, KF-406LHQ x 2 (4.6mmI.D. x 250mm each)
Eluent       : THF              
Flow rate    : 0.3mL/min
Column temp. : 25°C

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