The effect of injection volume on theoretical plate numbers (TPN) is investigated for GPC KF-402HQ, an organic SEC semi-micro column. Injection volume of 5 µL or less is appropriate for KF-402HQ.
Sample : Dicyclohexyl phthalate
Column : Shodex GPC KF-402HQ (4.6 mm I.D. x 250 mm each) Eluent : THF Flow rate : 0.3 mL/min Column temp. : 25 °C
Sample Name Index
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