The general flow rate of a semi-micro column (4.6 mm I.D.) is 0.3 mL/min. The eluent can pass through HK-404L column at a higher flow rate with the conventional HPLC instrument. Polystyrene was analyzed using a flow rate of 0.3 mL/min and 1.0 mL/min. When the flow rate was raised to 1.0 mL/min, the molecular chains were not degraded.


Sample : Polystyrene

Column       : Shodex GPC HK-404L (4.6 mm I.D. x 150 mm)
Eluent       : THF
Detector     : UV (254 nm) (small cell volume)
Column temp. : 40 °C