Aqueous/Organic (multi-solvent use) SEC columns, Asahipak GF-HQ series can be used with various kinds of eluent. The comparison of polystyrene separation with THF, chloroform and DMF eluent is shown below.
(Caution) Be careful that frequent replacement of in-column solvent may affect column life.


Sample : 1 mg/mL each, 50 μL
1. PS, Polystyrene (MW: 1,090,000)
2. PS, Polystyrene (MW: 190,000)
3. PS, Polystyrene (MW: 37,900)
4. PS, Polystyrene (MW: 9,100)
5. PS, Polystyrene (MW: 2,980)
6. PS, Polystyrene (MW: 500)

Columns      : Shodex Asahipak GF-510 HQ + GF-310 HQ (7.5 mm I.D. x 300 mm each)
Eluent       : (A): THF, (B): Chloroform, (C): DMF
Flow rate    : 0.5 mL/min
Detector     : (A),(B): UV (254 nm) (C): UV (270 nm)
Column temp. : 30 °C