Polystyrene standards were analyzed using GPC HK-403, an organic SEC (GPC) column for ultra-rapid analysis. The RI chromatograms showed that n-propylbenzene and benzene peaks overlapped with eluent related negative peak.
RI
Sample : 0.5 % each, 5 µL
1. Polystyrene (Mp: 139,000)
2. Polystyrene (Mp: 53,300)
3. Polystyrene (Mp: 21,800)
4. Polystyrene (Mp: 12,000)
5. Polystyrene (Mp: 9,860)
6. Polystyrene (Mp: 6,940)
7. Polystyrene (Mp: 3,180)
8. Polystyrene (Mp: 1,270)
9. Polystyrene (Mp: 580)
10. n-Propylbenzene (MW: 120)
11. Benzene (MW: 78)
UV
Sample : 0.1 % each, 5 µL
1. Polystyrene (Mp: 139,000)
2. Polystyrene (Mp: 53,300)
3. Polystyrene (Mp: 21,800)
4. Polystyrene (Mp: 12,000)
5. Polystyrene (Mp: 9,860)
6. Polystyrene (Mp: 6,940)
7. Polystyrene (Mp: 3,180)
8. Polystyrene (Mp: 1,270)
9. Polystyrene (Mp: 580)
10. n-Propylbenzene (MW: 120)
11. Benzene (MW: 78)
Columns : Shodex GPC HK-403 (4.6 mm I.D. x 150 mm) x 2 Eluent : THF Flow rate : 1.0 mL/min Detector : RI (small cell volume), UV (254 nm) (small cell volume) Column temp. : 25 °C
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