GPC HK-400 series, organic SEC (GPC) columns for ultra-rapid analyses, can be used with conventional HPLC systems as the column backpressure remains low even at high flow rate conditions. However, column pressure may become relatively high depending on the flow rate applied. Therefore, please pay an extra attention for the pump (system) pressure limit especially when multiple columns are used in series.
In this application, the effects of flow rate on the column pressure were studied under THF eluent condition. Please note that the values are for reference only as there are column-to-column and/or lot-to-lot variations. Also, note that the column pressure of guard column, HK-G, is not influenced by the flow rate, and thus does not add much pressure to the system.
Columns : Shodex GPC HK-400 series (4.6 mm I.D. x 150 mm each), HK-G Eluent : THF Flow rate : 0.3 mL/min, 0.6 mL/min, 1.0 mL/min Column temp. : 25 °C
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- GPC/MALS Analysis of Standard Polystyrene (KF-806L)
- Polystyrene Standards (1) (KF-801)
- Polystyrene Standards (2) (KF-802)
- Polystyrene Standards (3) (KF-803L)
- Polystyrene Standards (4) (KF-804L)
- Polystyrene Standards (5) (KF-805L)
- Polystyrene Standards (6) (KF-806L)
- Polystyrene Standards (7) (KF-807L)
- Polystyrene Standards (21) (GF-HQ : THF, Chloroform and DMF)
- Polystyrene Standards (23) (HK-404L)
- Polystyrene Standards (24) (HK-401)
- Polystyrene Standards (25) (HK-402)
- Polystyrene Standards (26) (HK-403)
- Poly(Ethylene Glycol) Standards (1) (THF Eluent) (KF-801)
- Molecular Weight Distribution of PMMA for Artificial Tooth (Comparison between Zimm Plot and GPC/MALS) (KF-806L)
- Poly(Methyl Methacrylate) Standards (1) (KF-805L)
- Poly(Methyl Methacrylate) Standards (2) (KF-806L)
- Polycarbonate Resin (1) (KF-806L)
- Polycarbonate Resin (3) (LF-804)
- Phenoxy Resin (1) (KF-806L)
- Phenoxy Resin (4) (LF-804)
- Phenol Resin (1) (KF-804L)
- Polycaprolactone (KF-806L)
- Poly(Vinyl Formal) (1) (KF-806L)
- Poly(Vinyl Formal) (2) (LF-804)
- 1,2-Polybutadiene (1) (KF-805L)
- 1,2-Polybutadiene (2) (LF-404)
- Poly(Vinyl Butyral) (1) (KF-806L)
- Poly(Vinyl Butyral) (2) (LF-804)
- Cellulose Acetate (3) (KF-806L)
- Cellulose Acetate Butyrate (KF-806L)
- Poly(Vinyl Chloride) (KF-806M)
- Poly(Vinyl Acetate) (KF-805L)
- Ethylene Vinyl Acetate Copolymer (1) (KF-806L)
- Ethylene Vinyl Acetate Copolymer (3) (LF-404)
- Poly(Butyl Methacrylate) (KF-806L)
- Poly(Butyl Methacrylate) (2) (HK-404L)
- Poly(Isobutyl Methacrylate) (KF-806L)
- Butyl Methacrylate Isobutyl Methacrylate Copolymer (KF-806L)
- Vinylidene Chloride Vinyl Chloride Copolymer (KF-805L)
- Vinyl Chloride Vinyl Acetate Vinyl Alcohol Terpolymer (KF-805L)
- Styrene butyl methacrylate copolymer (1) (KF-805L)
- Styrene Butyl Methacrylate Copolymer (2) (LF-404)
- Styrene Butyl Methacrylate Copolymer (3) (HK-404L)
- Styrene Butadiene ABA Block Copolymer (KF-806M)
- Styrene Isoprene ABA Block Copolymer (KF-806M)
- Styrene Ethylenre Butylene ABA Block Copolymer (KF-806M)
- Poly(2,4,6-tribromostyrene) (KF-805L)
- Polysulfone Resin (KF-805L)
- Poly(Propylene Glycol) (KF-802.5)
- Comparison of KF-402HQ and KF-802
- Polystyrene Oligomer Separation (KF-402HQ)
- Effects of Flow Rate (1) (KF-402HQ)
- Effects of Flow Rate (2) (KF-402.5HQ + KF-403)
- Effects of Injection Volume (KF-402HQ)
- Effects of Sample Concentration (1) (KF-402HQ)
- Effects of Sample Concentration (2) (KF-403HQ + KF-402HQ)
- Effects of Temperature (KF-402HQ)
- Effects of Instrument Settings on the Semi-micro Column Performance (KF-402HQ)
- Separation of Polystyrene Standard using GPC Columns
- Comparison of LF-804, LF-404 and LF-604
- Poly(Methyl Methacrylate) (1) (LF-404)
- Poly(Methyl Methacrylate) (3) (HK-404L)
- Analysis of PMMA in reference to USP-NF GENERAL CHAPTERS “Molecular Weight Determination for Copolymers Containing Alkyl Methacrylate or Alkyl Acrylate” (LF-804)
- Effects of Column Unit on Separation (1) (LF-404: PS)
- Effects of Column Unit on Separation (2) (LF-404: Epoxy Resin)
- Polyurethane (LF-404)
- Comparison of HK-404L and KF-805L
- Effects of Flow Rate (1) (HK-404L)
- Effects of Flow Rate (2) (HK-404L)
- Epoxy Resin (4) (Comparison of HK-404L and HK-404L + HK-401)
- Epoxy Resin (5) (Comparison of HK and KF-800 Series Columns)
- Epoxy Resin (6) (Comparison of HK-402 and KF-802.5)
- Epoxy Resin (7) ( Effects of Flow Rate for HK-402)
- Biodegradable Polymer (4) (Polylactic Acid) (HK-404L)
- Absolute Molecular Weight Determination of Star-shaped Polystyrene (HK-404L)
- Effects of Column Unit on Separation (HK-401: PS)
- Effects of Column Unit on Separation (HK-404L: PS)
- Effects of Column Unit on Separation (HK-406: PS)
- Effects of Flow Rate on Separation (HK-401: PS)
- Effects of Flow Rate on Separation (HK-404L: PS)
- Effects of Flow Rate on Separation (HK-406: PS)
- Comparison of Separation using HK-404L with Conventional Device and Semi-micro Type Device (HK-404L)
Applications (Related Information)
- Calibration Curves for KF-800 Series (THF Eluent: PS)
- Calibration Curves for KF-800L Series (THF Eluent: PS)
- Calibration Curves for KF-400HQ Series (THF Eluent: PS)
- Calibration Curve for HK-400 series (THF Eluent : PS)
- Calibration Curve for LF-804 (THF Eluent: PS)
- Calibration Curves for LF-404 and LF-604 (THF Eluent: PS)
- Calibration Curve for GF-510 HQ + GF-310 HQ (THF Eluent: PS)
- Calibration Curves for GF-HQ Series (THF Eluent: PS)