Effects of Column Unit on Separation (1) (LF-404: PS)

Each one, two and three units of GPC LF-404, were used for the analysis of the mixture of standard polystyrene compounds. Only a single unit of LF-404 can determine a broad range of molecular weights from 300 to 2,000,000. However the resolution is higher depending on the number of columns, so it is better to use several units of columns for obtaining more accurate molecular weight distribution.

Sample : 10 μL
1. PS, Polystyrene (MW: 1,030,000)
2. PS, Polystyrene (MW: 152,000)
3. PS, Polystyrene (MW: 66,000)
4. PS, Polystyrene (MW: 22,000)
5. PS, Polystyrene (MW: 5,050)
6. PS, Polystyrene (MW: 580)
7. Ethylbenzene

Columns      : Shodex GPC LF-404 (4.6 mm I.D. x 250 mm) x n
Eluent       : THF
Flow rate    : 0.3 mL/min
Detector     : RI (small cell volume)
Column temp. : 40 °C

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