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Effects of The Unit of Columns on The Separation (1) (LF-404: PS)


Each one, two and three units of GPC LF-404, were used for the analysis of the mixture of standard polystyrene compounds. Only a single unit of LF-404 can determine a broad range of molecular weights from 300 to 2,000,000. However the resolution is higher depending on the number of columns, so it is better to use several units of columns for obtaining more accurate molecular weight distribution.

Sample : 10μL
1. PS, Polystyrene (MW: 1,030,000)
2. PS, Polystyrene (MW: 152,000)
3. PS, Polystyrene (MW: 66,000)
4. PS, Polystyrene (MW: 22,000)
5. PS, Polystyrene (MW: 5,050)
6. PS, Polystyrene (MW: 580)
7. Ethylbenzene

Columns      : Shodex GPC LF-404 (4.6mmI.D. x 250mm) x n
Eluent       : THF
Flow rate    : 0.3mL/min
Detector     : Shodex RI(small cell volume)
Column temp. : 40°C

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