Effects of Flow Rate on Separation (HK-404L: PS)

Polystyrene standards were analyzed using GPC HK-404L, an organic SEC (GPC) column for ultra-rapid analysis. Comparing the separation between a HK-404L with a conventional SEC column of equivalent at the flow rate of 1.0 mL/min, HK-404L achieved the same level of separation with 1/6 analysis time. However, when higher separation is desired, use of lower flow rate is recommended.

Sample : 0.5 %, 5 µL
1. Polystyrene (Mp: 55,100)
2. Polystyrene (Mp: 7,210)
3. Polystyrene (Mp: 580)

Columns      : Shodex GPC HK-404L (4.6 mm I.D. x 150 mm) x 2
Eluent       : THF
Flow rate    : 1.0 mL/min, 0.6 mL/min, 0.3 mL/min
Detector     : RI (small cell volume)
Column temp. : 30 °C

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