Saccharides and amino sugars were analyzed using SUGAR SC1011, a column for analysis of saccharides. Generally, columns used for ligand exchange chromatography are used with water as the eluent, but adding cations as the counter ion enables the columns to separate samples under ion exchange modes well. Ca(NO₃)₂ aqueous solution was used as an eluent for SUGAR SC1011 with the counter ion as Ca²⁺, allowign ligand exchange and ion exchange to occur. Ligand exchange occurred between the counter ions of the packing material and the hydroxyl groups of saccharides. Ion exchange occurred between the sulfo groups and the amino groups of the saccharides. For sensitive measurement, attention should be paid to the positions of vacant peak due to the cations in the eluent.


Sample : 0.5 % each, 10 µL
1. Chloride anion
2. Sucrose
3. Glucose
4. Galactose
5. Mannose
6. D-Glucosamine
7. D-Galactosamine

Column       : Shodex SUGAR SC1011 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Ca(NO3)2 aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 80 °C