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Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD


The evaporative light scattering detector (ELSD) is a detector that measures the scattering of light of particles. This is achieved by evaporating the eluent eluted from the column, converting the eluate into particles and irradiating the particles with laser. Samples of interest include sugars and lipids and are used for applications similar to with a differential refractive index detectors (RID), but with ELSD a gradient elution analysis is possible and it is more sensitive. On the other hand, if there is a lot of bleed from the column, background noise increases and there is a possibility that sensitivity drops. Here, we compare the detection sensitivity of sugar alcohols by ELSD using polymer-based amino column Asahipak NH2P-50 4E and silica-based amino column. Compared with silica-based amino column, NH2P-50 4E has less column bleed, and it shows that the detection sensitivity is high with a larger signal-to-noise (S/N) ratio for all sugar alcohol peaks.

Sample : 20 μL
40 µg/mL each
(in 50 % CH3CN)
1. meso-Erythritol
2. Xylitol
3. Sorbitol
4. Maltitol

**(Note): Since ELSD's signal response has an exponential function instead of linear function, bleed noise amplifies each signal's peak exponentially which results in higher peak heights for the eluates for the silica-based columns. However, the signal-to-noise ratio is low in this case compared to the polymer-based columns where bleed noise is less.

Columns      : Shodex Asahipak NH2P-50 4E, Silica based amino column 
               (4.6 mm I.D. x 250 mm each)
Eluent       : CH3CN/H2O=80/20
Flow rate    : 1.0 mL/min
Detector     : ELS
Column temp. : 30 °C

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