Japanese Notification Eishin No. 47* (May 23, 1996, Ministry of Health, Labour and Welfare (MHLW)) “Analytical Methods for Nutritional Components, Following the Introduction of Nutrition Labeling Standards” includes “Appendix: Analytical Methods for Nutritional Components”. Under section 7. Sugars, monosaccharides or disaccharides that are not sugar alcohols are classified as sugars. (2) High-Performance Liquid Chromatography Method is divided into two parts. 1) Monosaccharides, Disaccharides, and Oligosaccharides and 2) Sugar Alcohols. Under 2) Sugar Alcohols, columns packed with silica gel modified with aminopropyl groups (polymer-based amino columns are also acceptable) or columns packed with sulfonated polystyrene gel (Pb type or Ca type) are used. In this application, SUGAR SP0810, a Pb type ligand-exchange column, was used to analyze standards of common sugars and sugar alcohols contained in foods.

*The version at the time of the application acquisition.

Sample: 5 µL
1 % each

1. 1.Sucrose
2. 2.Glucose
3. 3.Xylose
4. 4.Galactose
5. 5.Fructose
6. 6.meso-Erythritol
7. 7.Lactitol
8. 8.Mannitol
9. 9.Xylitol
10. 10.Sorbitol