Apple Juice (NH2P-50 4E)

When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before the analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E is shown together with the sample pre-treatment procedure.

(Sample pre-treatment for apple juice)
1) Put 5 g of apple juice in a 50 mL a volumetric flask.
2) Dilute with ultra pure water to make up a 30 mL solution.
3) Neutralize with 10 %(W/V) NaOH solution.
4) Apply ultrasonic vibration for 30 minutes using an ultrasonic vibrator.
5) Add ultra pure water to make up a 50 mL solution.
6) Filtrate the solution with a paper filter (No.5B).
7) Take a part of filtrated solution and add the same volume of acetonitrile.
8) Pass through a 0.45 μm membrane filter before injecting into HPLC.


Sample : Apple juice, 20 μL
1. Fructose
2. Sorbitol
3. Glucose
4. Sucrose

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : Room temp.(25 °C)