Analysis of Sugar Alcohols According to Japanese Food Labeling Standards (Former Nutrition Labeling Standards) (NH2P-50 4E)

Japanese Notification Eishin No. 47* (May 23, 1996, Ministry of Health, Labour and Welfare (MHLW)) “Analytical Methods for Nutritional Components, Following the Introduction of Nutrition Labeling Standards” includes “Appendix: Analytical Methods for Nutritional Components”. Under section 7. Sugars, monosaccharides or disaccharides that are not sugar alcohols are classified as sugars. (2) High-Performance Liquid Chromatography Method is divided into two parts. 1) Monosaccharides, Disaccharides, and Oligosaccharides and 2) Sugar Alcohols. Under 2) Sugar Alcohols, columns packed with silica gel modified with aminopropyl groups (polymer-based amino columns are also acceptable) or columns packed with sulfonated polystyrene gel (Pb type or Ca type) are used. In this application, Asahipak NH2P-50 4E, a polymer-based amino column, was used to analyze standards of common sugars and sugar alcohols contained in foods. A mixed solvent of water and acetonitrile was used as the mobile phase, and by adjusting the mixing ratio, the elution of the sample could be controlled. Polymer-based amino columns exhibit less change over time compared to silica-based amino columns and can also be cleaned with alkaline solutions.

(note)
On April 1, 2015, the Food Labeling Act was enforced, and the Nutrition Labeling Standards were integrated into the Food Labeling Standards.
 
On March 30, 2022, the “Appendix: Analytical Methods for Nutritional Components” was revised, and the measurement of sugar alcohols was moved from 7. Sugars to 35. Energy.

*The version at the time of the application acquisition.

Sample: 20 µL
0.5 % each

  1. 1.meso-Erythritol
  2. 2.Xylitol
  3. 3.Sorbitol
  4. 4.myo-Inositol
  5. 5.Maltitol
  6. 6.Palatinit
Column
：Shodex Asahipak NH2P-50G 4A (4.6 mm I.D. x 10 mm)+ NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
：H2O/CH3CN=25/75
Flow rate
：1.0 mL/min
Detector
：RI
Column temp.
：Room temp.




Sample : 0.5 % each, 20 μL
1. meso-Erythritol
2. Xylitol
3. Sorbitol
4. myo-Inositol
5. Maltitol
6. Palatinit

  
Column       : Shodex Asahipak NH2P-50G 4A (4.6 mm I.D. x 10 mm)
               + NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : H2O/CH3CN=25/75
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : Room temp.

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