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Separation of myo-Inositol and D-chiro-Inositol (SZ5532)

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There are nine kinds of naturally occurring inositol isomers and myo-inositol that have been used as aprophylactic or therapeutic drug for fatty liver and cirrhosis. Otherwise, D-chiro-inositol has been confirmed various physiological functions such as improvement of non-insulin-dependent diabetes mellitus and polycystic ovary disease. In this application, myo-inositol and D-chiro-inositol were separated using SUGAR SZ5532, a column for sugar analysis.


Sample : 0.5 % each, 10 µL
1. D-chiro-Inositol
2. myo-Inositol


Column       : Shodex SUGAR SZ5532 (6.0 mm I.D. x 150 mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 60 °C

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