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Apple Juice (SC1011)

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Saccharides and a sugar alcohol contained in apple juice were analyzed using SUGAR SC1011 (a column for saccharides analysis).

(Sample pretreatment)
1) Take 5 g of apple juice in a beaker, add 30 mL of pure water and neutralize with 10 %(W/V) NaOH aq. cooled with ice.
2) Apply ultrasonic vivration for 30 minutes and make 50mL solution with pure water.
3) Filtrate with 0.45 μm membrane filter.


Sample : Apple juice 5 μL
1. Sucrose
2. Glucose
3. Fructose
4. Sorbitol

Column       : Shodex SUGAR SC1011 (8.0 mm I.D. x 300 mm)
Eluent       : H2O
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : 85 °C

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