Xylitol is officially permitted as a food additive by the Ministry of Welfare in Japan on April 17, 1997. The chromatogram is an analysis of xylitol in tablet candy. Asahipak NH2P-50 4E column is quite useful for the analysis of sugar alcohols including xylitol. NH2P-50 is a polymer-based amino column, and packed with the polyvinylalcohol gel bonded with polyamine. It is good for quantitative analysis and can be washed with alkaline solvents.
(Sample pretreatment)
2 % aqueous solution of the sample was passed through a 0.45 μm filter,
and the same volume of acetonitrile was added.

Sample : Tablet candy, 20 μL
1. Xylitol
2. Sorbitol
(Sample pretreatment)
2 % aqueous solution of the sample was passed through a 0.45 μm filter,
and the same volume of acetonitrile was added.

Sample : Tablet candy, 20 μL
1. Xylitol
2. Sorbitol
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : H2O/CH3CN=25/75 Flow rate : 1.0 mL/min Detector : RI Column temp. : 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides and Sugar Alcohols (NH2P-50 4E)
- Sugar Alcohols (1) (SC1211)
- Sugar Alcohols (2) (NH2P-50)
- Sugar Alcohols (3)
- Saccharides and Sugar Alcohols (1) (SZ5532)
- Saccharides and Sugar Alcohols (2) (SP0810)
- Saccharides and Sugar Alcohols (3) (SP0810)
- Saccharides and Sugar Alcohols (4) (SC1011)
- Saccharides and Sugar Alcohols (5) (SZ5532)
- Saccharides and Sugar Alcohols (6) (NH2P-50)
- Saccharides and Amino Acids (1) (SC1011)
- Saccharides and Amino Acids (2) (SC1211)
- Saccharides and Amino Acids (3) (KS-801)
- Saccharides and Amino Sugars (2) (NH2P-50 4E)
- Saccharides and Amino Sugars (3) (SC1011)
- Apple Juice (NH2P-50 4E)
- Apple Juice (SC1011)
- Sake (Japanese Rice Wine)
- Effects of Acetonitrile Concentration in Analysis of Sugar Alcohols
- Amino Sugars (SB-802.5 HQ)
- Soft Drink Containing Erythritol
- Palatinit
- Analysis of Pinitol (SP0810)
- Analysis of Hydrogenated Maltose Starch Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Mannitol According to Pharmacopeia Method (SC1011-7F)
- Analysis of myo-Inositol According to EP Method (SC1011-7F)
- Analysis of Xylitol According to USP-NF Method (SP0810)
- Analysis of Meglumine (NN-814)
- LC/MS/MS Analysis of Meglumine (VC-50 2D)
- Analysis of Meglumine (YS-50)
- Separation of myo-Inositol and D-chiro-Inositol (SZ5532)
- Separation of Taurine and Inositol (NH2P-50 4E)
- Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD
- Analysis of Erythrose and Erythritol (VG-50 4E)