Lipoxidase, an oxidoreductase was analyzed using IEC SP-825 (a column for strong anion exchange chromatography). It is a glycoprotein of MW 80,000. Lipoxidase from soybean consists of three fractions. Enzyme activity was found at two peaks around 25 minutes.


Sample: 0.5 % Lipoxidase, 150 μL

Column       : Shodex IEC SP-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Citric acid-NaOH buffer (pH4.3)
               (B); (A) + 0.5 M Na2SO4
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C