Chytochrome P450 in rat liver microsome was analyzed using Asahipak ES-502C 7C (a column for weak cation exchange chromatography).


Sample : Cytochrome P450 in rat liver microsome

Column       : Shodex Asahipak ES-502C 7C (7.5 mm I.D. x 100 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH6.5) + 20 % Glycerol + 0.4 % Emulgen 911
               (B); (A) + 1.0 M Sodium Acetate
               Linear gradient: 0 min to 50 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : VIS (417 nm)
Column temp. : Room temp.

Courtesy of Dr. Funae, Osaka City University