The tables show good recovery of IEC CM-825 (a column for weak cation exchange chromatography) for proteins and enzyme activity.
| Recovery of proteins | |
|---|---|
| Protein | Recovery(%) |
| α Chymotrypsinogen A | 90 |
| Myoglobin | 97 |
| Ribonuclease A | 100 |
| Cytochrome c | 101 |
| Lysozyme | 99 |
| Recovery of enzyme activity | |
|---|---|
| Enzyme | Recovery(%) |
| α Chymotrypsin | 97 |
| Lysozyme | 102 |
Sample load : 0.5 mg each
Column : Shodex IEC CM-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 20 mM Sodium phosphate buffer (pH7.0)
(B); (A) + 0.5 M NaCl
Linear gradient: 0 min to 1 min, (A) to (B)
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Recovery of Proteins and Enzime Activity (QA-825)
- Recovery of Proteins and Enzyme Activity (DEAE-825)
- Recovery of Proteins and Enzyme Activity (SP-825)
- Effects of Sample Load on Resolution (QA-825)
- Effects of Sample Load on Peak Width (DEAE-825)
- Effects of Sample Load on Peak Width (SP-825)
- Effects of Sample Load on Resolution (CM-825)
- Effects of pH on Elution Pattern (DEAE-825)
- Effects of pH on Elution Pattern (SP-825)
- Standard Proteins (5) (QA-825)
- Standard Proteins (6) (ES-502N 7C)
- Standard Proteins (7) (CM-825, SP-825)
- Standard Proteins (8) (ES-502C 7C)
- Crude Albumin (Chicken Egg)
- Human Serum (4) (DEAE-825)
- Proteins in Human Serum (1) (QA-825)
- Proteins in Human Serum (2) (DEAE-825)
- Lipoxidase from Soybean (1) (QA-825, DEAE-825)
- Collagen and BSA
- β Lactoglobulin Subfractions
- Angiotensin Ⅰ, Ⅱ, Ⅲ
- Lysozyme Chloride
- Cytochrome P450 in Rat Liver Microsome
- Myosin Subfragments
- Bovine Liver Catalase
- Purified Kallikrein from Pig Pancreas
- Ribonuclease A from Equine Pancreas (CM-825)
- Lipoxidase (1) (SP-825)
- Papain
- Mercaptpalbumin and Nonmercaptoalbumin by Ion Exchange Chromatography