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Recovery of Proteins and Enzyme Activity (CM-825)

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The tables show good recovery of IEC CM-825 (a column for weak cation exchange chromatography) for proteins and enzyme activity.

Recovery of proteins
ProteinRecovery(%)
α Chymotrypsinogen A 90
Myoglobin 97
Ribonuclease A 100
Cytochrome c 101
Lysozyme 99
Recovery of enzyme activity
EnzymeRecovery(%)
α Chymotrypsin 97
Lysozyme 102


Sample load : 0.5 mg each

Column : Shodex IEC CM-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 20 mM Sodium phosphate buffer (pH7.0)
         (B); (A) + 0.5 M NaCl
         Linear gradient: 0 min to 1 min, (A) to (B)

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