The tables show good recovery of IEC DEAE-825 (a column for ion exchange chromatography) for proteins and enzyme activity.
Sample load : 0.5 mg each
Recovery of proteins | |
---|---|
Protein | Recovery (%) |
Trypsin inhibitor | 96 |
β-Lactoglobulin | 100 |
Ovalbumin | 101 |
Myoglobin | 100 |
γ-Globulin | 99 |
BSA | 97 |
Thyroglobulin | 90 |
Recovery of enzyme activity | |
---|---|
Enzyme | Recovery (%) |
Kallikrein | 92 |
Lipoxidase | 96 |
Catalase | 94 |
Sample load : 0.5 mg each
Column : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm) Eluent : (A); 20 mM Tris-HCl buffer (pH8.0) (B); (A) + 0.5 M NaCl Linear gradient: 0 min to 1 min, (A) to (B)
Sample Name Index
Applications
- Recovery of Proteins and Enzime Activity (QA-825)
- Recovery of Proteins and Enzyme Activity (SP-825)
- Recovery of Proteins and Enzyme Activity (CM-825)
- Effects of Sample Load on Resolution (QA-825)
- Effects of Sample Load on Peak Width (DEAE-825)
- Effects of Sample Load on Peak Width (SP-825)
- Effects of Sample Load on Resolution (CM-825)
- Effects of pH on Elution Pattern (DEAE-825)
- Effects of pH on Elution Pattern (SP-825)
- Standard Proteins (5) (QA-825)
- Standard Proteins (6) (ES-502N 7C)
- Standard Proteins (7) (CM-825, SP-825)
- Standard Proteins (8) (ES-502C 7C)
- Crude Albumin (Chicken Egg)
- Human Serum (4) (DEAE-825)
- Proteins in Human Serum (1) (QA-825)
- Proteins in Human Serum (2) (DEAE-825)
- Lipoxidase from Soybean (1) (QA-825, DEAE-825)
- Collagen and BSA
- β Lactoglobulin Subfractions
- Angiotensin Ⅰ, Ⅱ, Ⅲ
- Lysozyme Chloride
- Cytochrome P450 in Rat Liver Microsome
- Myosin Subfragments
- Bovine Liver Catalase
- Purified Kallikrein from Pig Pancreas
- Ribonuclease A from Equine Pancreas (CM-825)
- Lipoxidase (1) (SP-825)
- Papain
- Mercaptpalbumin and Nonmercaptoalbumin by Ion Exchange Chromatography