Angiotensins were analyzed using IEC SP-825 (a column for strong cation exchange chromatography). Angiotensins are physically active peptides and cause blood pressure to rise. Angiotensin Ⅰ is a decapeptide, Angiotensin Ⅱ an octapeptide and Angiotensis Ⅲ a hexapeptide. These three components separated very well by gradient elution with increasing salt concentration.


Sample : 250 μL
1. Angiotensin Ⅱ
2. Angiotensin Ⅰ
3. Angiotensin Ⅲ

Column       : Shodex IEC SP-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH7.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.