Proteins in human serum were analyzed using IEC DEAE-825 (a column for weak anion exchange chromatography).


Sample : Human Serum
1. Albumin
2. α1 Acid glycoprotein
3. Transferrin
4. IgA
5. IgM
6. IgG

Column       : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Tris-CH3COOH buffer (pH8.0)
               (B); (A) + 0.5 M CH3COONa
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.