Papain was analyzed using IEC SP-825 (a column for strong cation exchange chromatography). Papain is a polypeptide chain of MW 23,406 and pI 8.75. It is very stable protelytic enzyme. It is used as the reagent for the study of structure of proteins and also for industrial use.


Sample : Papain

Column       : Shodex IEC SP-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH7.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C