Proteins in Human Serum (1) (QA-825)

Proteins in Human serum were analyzed using IEC QA-825 ( a column for strong anion exchange chromatography ).
In this analysis, the sample was separated into four peaks including the transferrin peak; the largest one is serum albumin.


Sample : Human serum
1. IgG
2. Transferrin
3. (Unknown)
4. Serum albumin

Column       : Shodex IEC QA-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Tris-HCl buffer (pH8.6)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 60 min, 100 % (A) to 50 % (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.

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