Collagen and BSA were analyzed using Asahipak ES-502N 7C (a column for weak anion exchange chromatography).


Sample :
1. Collagen
Pepsin-digested product of collagen
soluble in acid extracted from pig skin
2. BSA

Column       : Shodex Asahipak ES-502N 7C (7.5 mm I.D. x 100 mm)
Eluent       : 20 mM Monoethanolamine buffer (pH9.5) + 500 mM NaCl 
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

Courtesy of Dr. Shirai, Tokyo University of Agriculture and Technology