β Lactoglobulin Subfractions

β Lactoglobulin subfractions were analyzed using Asahipak ES-502N 7C (a column for weak anion exchange chromatography).


Sample : β-Lactoglobulin

Column       : Shodex Asahipak ES-502N 7C (7.5 mm I.D. x 100 mm)
Eluent       : (A); 20 mM Piperazine (pH5.0)
               (B); (A) + 300 mM NaCl
               Linear gradient: 0 min to 12 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

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